Formulation and Antioxidant Activity Test of Emulgel Extract of Guava (Psidium Guajava Linn.) Using DPPH (2,2-Diphenyl-1- Picrylhydrazyl) Methods
DOI:
https://doi.org/10.70075/jftsk.v3i1.291Keywords:
antioxidant, emulgel, guava, extract, DPPHAbstract
Plants that have the potential to contain antioxidants are guava leaves (Psidium guajava Linn.) which contain one of the compounds, flavonoids, which have antioxidant activity that can reduce free radicals. Emulgel has advantages such as thixotropic, easy to spread, easy to clean, does not leave stains, durable and can be a delivery system for hydrophobic and hydrophilic substances. This study aims to formulate guava leaf extract emulgel and determine antioxidant activity using DPPH method. This research began with guava leaf extraction and base optimization with varying concentrations of stearic acid as an emulsifier consisting of F1 8%, F2 10% and F3 12%. The base that meets the requirements of good physical stability is F2. F2 base was made into emulgel preparations with 3 variations of guava leaf extract concentration, namely F2a 1%, F2b 2% and F2b 3%. The three formulas were tested for physical stability including organoleptic test, emulsion type, pH test, spreadability, adhesiveness, viscosity test, and freeze thaw test as well as antioxidant activity test with DPPH method. The results of the physical stability test showed that the preparations F2a, F2b and F2c met the requirements. The IC50 value in each formulation has a different antioxidant activity value. F2a formulation is 123.59 ppm and F2b is 107.08 ppm which is classified as moderate antioxidant activity, while F2c is 61.74 ppm which is classified as strong antioxidant activity. Guava leaf extract emulgel formula, especially the second formula (F2c), has great potential to be considered by the pharmaceutical industry to be developed into antioxidant products because the results of the evaluation that have been carried out meet the requirements in terms of physical evaluation and potential antioxidant strong.
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References
Narayanan DL, Saladi RN, Fox JL. Ultraviolet radiation and skin cancer 2010. https://doi.org/10.1111/j.1365-4632.2010.04474.x
Wei M, He X, Liu N, Deng H. Role of reactive oxygen species in ultraviolet ‑ induced photodamage of the skin. Cell Div 2024:1–9. https://doi.org/10.1186/s13008-024-00107-z
Morris G, Gevezova M, Sara V, Maes M. Redox regulation of the immune response 2022;1. https://doi.org/10.1038/s41423-022-00902-0
Chandimali N, Bak SG, Park EH, Lim H, Won Y, Kim E. Free radicals and their impact on health and antioxidant defenses : a review. Cell Death Discov 2025. https://doi.org/10.1038/s41420-024-02278-8
Rudrapal M, Rakshit G, Singh RP, Garse S, Khan J. Dietary Polyphenols : Review on Chemistry / Sources , Bioavailability / Metabolism , Antioxidant Effects , and Their Role in Disease Management 2024. https://doi.org/10.3390/antiox13040429
Michalak M. Plant-Derived Antioxidants : Significance in Skin Health and the Ageing Process 2022:8–12. https://doi.org/10.3390/ijms23020585
Rudenko NN, Vetoshkina D V, Marenkova T V, Borisova-mubarakshina MM. Antioxidants of Non-Enzymatic Nature : Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis 2023. https://doi.org/10.20944/preprints202310.2051.v1
Biochem A, Pisoschi AM, Negulescu GP. Biochemistry & Analytical Biochemistry Methods for Total Antioxidant Activity Determination : A Review 2011;1:1–10. https://doi.org/10.4172/2161-1009.1000106
Huynh HD, Nargotra P, Wang HD, Shieh C, Liu Y, Kuo C. Bioactive Compounds from Guava Leaves ( Psidium guajava L .): Characterization , Biological Activity , Synergistic Effects , and Technological Applications 2025. https://doi.org/10.3390/molecules30061278
Khanna S, Singh P. Comparative Analysis of Bioactive Compounds in Guava ( Psidium guajava L .) Fruit and Leaves 2025;15:53–8. https://doi.org/10.9734/ajrb/2025/v15i2366
Profile P, Bioactivities H. Guava ( Psidium guajava L .) Leaves : Nutritional Composition , 2021:1–20.
Bulugahapitiya VP, Kokilananthan S, Manawadu H, Sanath C. Phytochemistry and medicinal properties of Psidium guajava L . leaves : A review 2021;8:963–71. https://doi.org/10.14719/pst.2021.8.4.1334
Sabalingam S, Siriwardhene MA. A review on emerging applications of emulgel as topical drug delivery system 2022.
Padhan A. Emulgel : A Novel Approach For Topical Drug Delivery 2025:5336–42.
Vishwakarma G, Panwar AS. Emulgel Emergent Systems : At A Glance For Topical Drug Delivery 2022;15. https://doi.org/10.22159/ajpcr.2022.v15i3.43876
Jabbar MJ. Emulgel Considered as Anovel Type of Dosage Form for Topical Application 2024;22:4551–60. https://doi.org/10.57239/PJLSS-2024-22.1.00335
Malavi S, Kumbhar P, Manjappa A, Chopade S, Patil O, Kataria U, et al. Topical Emulgel : Basic Considerations in Development and Advanced Research 2022;84:1105–15. https://doi.org/10.36468/pharmaceutical-sciences.1005
Pooja J, Amir DS, Rahul DB. Emulgel Review – A Novel Topical Drug Delivery System. Int J Pharm Sci Rev Res 2024;84:115–21. https://doi.org/10.47583/ijpsrr.2024.v84i05.020
Vasconcelos AG, Amandda É, Carvalho S De, Slompo RC, S BS, Maria J, et al. Lycopene from Red Guava ( Psidium guajava L .): From Hepatoprotective Effect to Its Use as Promising Self-Emulsifying Drug Delivery System for Anti-Inflammatory and Antioxidant Applications 2023.
Luo Y, Peng B, Wei W, Tian X. Antioxidant and Anti-Diabetic Activities of Polysaccharides from Guava Leaves 2019:1–14. https://doi.org/10.3390/molecules24071343
Faradilla M, Rizal K. Phytochemical screening analysis of Guava leaf extract ( Psidium guajava L . ) against the content of Saponins , Tannins , and Flavonoids 2023;9:117–26. https://doi.org/10.21580/jnsmr.2023.9.2.17835
Montiel DG, Lilian A, Barrera G, Cristian G, Mart G, Hernandez G, et al. Influence of the Extraction Method on the Polyphenolic Profile and the Antioxidant Activity of Psidium guajava L . Leaf Extracts 2024.
Setyawaty R, Setiyabudi R. Gel Formulation from Ethanol Extract of the Leaf of White Guava ( Psidium guajava L .) 2021;26:149–54. https://doi.org/10.22146/mot.59725. https://doi.org/10.22146/mot.59725
Mustapa MA, Taupik M, Mu A, Suryadi A, Paneo MA. Penentuan Kadar Flavonoid Daun Sembung ( Blumea balsamifer ) menggunakan Metode Spektrofotometri UV-Vis 2024;4:255–61. https://doi.org/10.37311/ijpe.v4i2.11284
Mustapa MA, Paneo MA, Puluhulawa LE, Latif MS, Nani I. Formulasi Dan Evaluasi Sediaan Exfoliating Gel Sari Buah Anggur ( Vitis vinifera L . ). J Farm Teknol Sediaan Dan Kosmet 2025;2:35–44. https://doi.org/10.70075/jftsk.v2i1.150
Mu A, Suryadi A, Paneo MA, Djuwarno EN, Balikan VA. Formulasi Dan Evaluasi Sediaan Emulgel Sabun Cuci Tangan dari Ekstrak Tomat ( Solanum Lycopersicum L .). J Farm Teknol Sediaan Dan Kosmet 2025;2:45–54. https://doi.org/10.70075/jftsk.v2i1.166
Paneo M.A, Thomas NA, Latif MS, Tuloli TS. Formulation and Physical Characterization of Emulgel Facial Wash Containing a Combination of Almond Oil ( Prunus amygdalus dulcis ) and Sodium Lauryl Sulfate Surfactant Formulasi dan Karakterisasi Fisik Sediaan Emulgel Facial wash dengan 2025;8:1318–27. https://doi.org/10.36490/journal-jps.com.v8i2.640
Amrin L, Aslam S, Nadu T. Research Article Formulation and Evaluation of Emulgel Containing Tridax Procumbens Extract 2023;79:176–80. https://doi.org/10.47583/ijpsrr.2023.v79i02.028
Thombre NA, Niphade PS, Ahire ED, Kshirsagar SJ. Formulation Development and Evaluation of Microemulsion Based Lornoxicam Gel 2022;19:69–80. https://doi.org/10.13005/bbra/2968
Djuwarno EN, Paneo MA. Formulasi Dan Evaluasi Sediaan Facial Scrub Sari Tebu ( Saccharum Officinarum Linn .) Sebagai Exfoliate Cleanser 2025;2:94–102. https://doi.org/10.70075/jftsk.v2i2.181
Paneo MA, Pakaya M S, Thomas NA, Moo FRC, Puluhulawa LE, Thamrin R, et al. Formulasi Ekstrak Kulit Jeruk Nipis (Citrus Aurantifolia) Dalam Masker Gel Peel-Off Anti Aging Dengan Metode DPPH (2,2-diphenyl-1-picrylhydrazyl). J Farm Teknol Sediaan Dan Kosmet 2024;1:11–20. https://doi.org/10.70075/jftsk.v1i1.6
Taupik M, Banne PZ, Paneo MA, Puluhulawa LE, Mustapa MA. Formulasi Dan Evaluasi Sediaan Sabun Under Arm Wash Dari Minyak Atsiri Daun Kemangi ( Ocimum sanctum L .) 2025;2:103–11. https://doi.org/10.70075/jftsk.v2i2.190
Tungadi R, Abdulkadir WS, Hiola F. Pengaruh SLM _ 2026 Terhadap Stabilitas Fisik dan Laju Pelepasan Krim Niosom Antosianin Secara In Vitro 2026;2:55–68. https://doi.org/10.70075/jftsk.v2i2.65
Mu A, Suryadi A, Paneo MA, Thomas N, Manno MR, Sabrina PF. Formulasi Dan Evaluasi Sedian Gel Sabun Cuci Tangan Ekstrak Jeruk Kalamansi ( Citrus Microcarpa ). J Farm Teknol Sediaan Dan Kosmet 2025;2:1–15. https://doi.org/10.70075/jftsk.v2i1.145
Castro-enríquez D, Miranda JM, Trigo M, Rodríguez-félix F, Aubourg SP, Barros-velázquez J. Efek Antioksidan dan Antimikroba dari Film Biodegradable Mengandung Ekstrak Pitaya ( Stenocereus thurberi ) selama Penyimpanan Ikan di Kulkas 2023.
Priani SE, Irawati I, Darma GCE. Formulation of Peel-Off Facial Mask from Mangosteen Pericarp (Garcinia mangostana Linn.). Indones J Pharm Sci Technol 2015;2:90–5. https://doi.org/10.15416/ijpst.v2i3.7905
Majhi R, Maharjan R, Shrestha M, Basnet A, Baral M, Duwal R. Effect of altitude and solvent on Psidium guajava Linn . leaves extracts : phytochemical analysis , antioxidant , cytotoxicity and antimicrobial activity against food spoilage microbes. BMC Chem 2023:1–16. https://doi.org/10.1186/s13065-023-00948-9
Taurina W, Andrie M. Characterization of the Manufacturing Process Simplicia Syzygium aromaticum as Raw Material for Wound Healing Drug Preparations n.d.;4:354–62.
Wulandini R, Irwansyah FS, Windayani N. Formulation of facial cleansing gels using aloe vera as natural surfactant. J Phys Conf Ser 2019;1402. https://doi.org/10.1088/1742-6596/1402/5/055069
Shimpi R. Relative Kinetic Study of Oxidation of Ethanol by Potassium Dichromate and Potassium Permanganate in Aqueous Acidic Media 2023;12:686–91. https://doi.org/10.21275/SR231207185752
Zhang P, Hai H, Sun D, Yuan W, Liu W, Ding R, et al. A high throughput method for total alcohol determination in fermentation broths 2019:1–8. https://doi.org/10.1186/s12896-019-0525-7
Hassan J, Mehdizadeh S, Kazem M, Pourshaban-shahrestani A, Zayerzadeh E. MethodsX A green chemical analysis of ethanol using a smart phone. MethodsX 2024;13:102809. https://doi.org/10.1016/j.mex.2024.102809
Paneo MA, Djuwarno EN, Habibie SA. Formulation and In Vivo Evaluation of Hulu ’ u Fish ( Giuris margariticus ) Albumin Spray Gel for Diabetic Wounds 2026;8:59–78. https://doi.org/10.37311/jsscr.v8i1.34957
Pomalango ZB, Paneo MA, Nora M, Siregar I, Sapiun Z, Lihawa K, et al. Poloxamer-188 Based Curcumin Nanoemulsion : Formulation , Characterization , And Stability For Entrapment Efficiency 2026;18. https://doi.org/10.22159/ijap.2026v18i2.57293
Paneo MA, Thomas N, Ramadhani FN, Latif MS, Moo FR, Puluhulawa LE, et al. Tropical Journal of Natural Product Research Innovative Gel Formulation with 70 % Ethanol Extract of Pandanus amaryllifolius Leaf for Accelerated Healing of Second-Degree Burn in Rats 2026;10:6493–503. https://doi.org/10.26538/tjnpr/v10i1.9
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, et al. Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of 2022. https://doi.org/10.3390/molecules27041326
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