Characterization of Methanol Fraction Compounds from Formulation Chinese Senna Leaves (Cassia Alata L.) Using UV-Vis Spectrophotometry Method
DOI:
https://doi.org/10.70075/jftsk.v2i3.143Keywords:
Spektrofotometri UV-Vis, Metabolit SekunderAbstract
The leaves of Cassia Alata L. (Chinese Senna) are an important part of daily human life and are used as medicine for treating ringworm, worms, constipation, and canker sores. Cassia Alata L., also known as Gelinggang, has a spicy taste and is warm in nature. This study aims to identify the secondary metabolite compounds present in Formula Cassia Alata L. leaves. The method used in this study is maceration with methanol as the solvent. Qualitative analysis in this study utilized color tests, thin-layer chromatography (TLC) with a hexane:ethyl acetate (4:1) solvent system, and quantitative analysis using UV-Vis spectrophotometry at wavelengths of 260.00 nm, 213.18 nm, 345.24 nm, 202.56 nm, and 363.63 nm. The results show that Cassia Alata L. leaves positively contain flavonoids, alkaloids, steroids, saponins, and tannins. It is concluded that the identification of secondary metabolite compounds from Cassia Alata L. leaves using UV-Vis spectrophotometry with methanol as a solvent provides spectra that correspond to secondary metabolite compoundsDownloads
References
Widodo H, Rohman A, Penelitian B, Tengah J, Bioteknologi PS, Pascasarjana S, et al. Pemanfaatan Tumbuhan Famili Fabaceae untuk Pengobatan Penyakit Liver oleh Pengobat Tradisional Berbagai Etnis di Indonesia 2018:65–88.
Pranaka RN, Yusro F, Budiastutik I. Di Kabupaten Sambas The Utilization of Medicinal Plants by Melayu Ethnic in Sambas Regency 2020;13:1–24.
Selegato DM, Monteiro AF, Vieira NC, Cardoso P, Pavani VD, Bolzani VS, et al. Update : Biological and Chemical Aspects of S enna spectabilis 2017;28:415–26.
Francesca I, Mera G, Estefanía D, Falconí G, Córdova VM. Secondary metabolites in plants : main classes , phytochemical analysis and pharmacological activities . 2019.
Zreen Z, Hameed A, Kiran S, Farooq T, Zaroog MS. Research Article A Comparative Study of Diospyros malabarica ( Gaub ) Extracts in Various Polarity-Dependent Solvents for Evaluation of Phytoconstituents and Biological Activities 2022;2022.
Akinmoladun AC, Falaiye OE, Ojo OB, Adeoti A. Effect of extraction technique , solvent polarity , and plant matrix on the antioxidant properties of Chrysophyllum albidum G . Don ( African Star Apple ). Bull Natl Res Cent 2022. https://doi.org/10.1186/s42269-022-00718-y.
Sharma S, Malik J, Chandra D. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Methanolic Extract of Spermacoce hispida 2022;0990:22–5. https://doi.org/10.36349/easjpp.2022.v04i01.003.
Rashed A, Shaeroun A, Ahmed AB, Alqamoudy H, Mohamed KS. Thin Layer Chromatography ( TLC ) and Phytochemical Analysis of Moringa Oleifera Methanol , Ethanol , Water and Ethyl Acetate Extracts 2019;4929. https://doi.org/10.36348/SJMPS.2019.v05i10.002.
Extraction T, Analysis C, Cassia L. Ekstraksi dan Analisis Kimia Daun Gulinggang ( Cassia alata Linn .) dengan Pelarut Air dan Etanol 2017:1–8.
Extract E, Bar BS. Pemanfaatan Ekstrak Etanol Daun Gulinggang ( Cassia alata Linn ) sebagai Bahan Antijamur pada Produk Sabun Mandi 2018.
Zabri H, Kodjo C, Benie A, Bekro JM, Alain Y. Phytochemical screening and determination of flavonoids in Secamone afzelii ( Asclepiadaceae ) extracts 2008;2:80–2.
Ladan Z, Amupitan JO, Oyewale OA, Ayo RG, Temple E, Ladan EO. Phytochemical screening of the leaf extracts of Hyptis spicigera plant 2014;8:83–8. https://doi.org/10.5897/AJPAC2014.
Rahmadi A, Zaidar E, Hardiyanti R, Zulham M. Phytochemical and toxicity test with the BSLT method on Kemuning Raya leaf extract ( Muraya paniculata L .) Phytochemical and Toxicity Test Z ith W he BSLT Method on Kemuning Raya Leaf Extract ( Muraya paniculata L .) 2021;080006.
Inalegwu B. Antimicrobial and foam forming activities of extracts and purified saponins of leaves of Tephrosia vogelii 2015;5:49–53.
Mailoa MN, Mahendradatta M, Laga A, Djide N. Tannin Extract Of Guava Leaves ( Psidium Guajava L ) Variation With Concentration Organic Solvents 2013;2:106–10.
Javier R, Hidalgo D, Córdoba R, Grigoryan H, Vieira M, Melo MJ, et al. The making of black inks in an Arabic treatise by al ‑ Qalalūsī dated from the 13th c .: reproduction and characterisation of iron ‑ gall ink recipes. Herit Sci 2023:1–14. https://doi.org/10.1186/s40494-022-00823-1.
Gambo NN, Okooboh MA, Ibejekwe SJ, Ogori BO, Chindo IY, Agada GOA. Phytochemical and Chromatographic Studies of the Polar Extracts of the Leaves of Cassia alata Linn . 2013;3:89–96.
Narayanan S, Jadhav AP. Simultaneous Estimation of Aloe Emodin and Emodin from Rheum emodi , Cassia alata and Aloes by HPTLC 2015;77:783–7.
Mukhopadhyay G, Jain D, Sodani A, Pramanick A, Das AK, Sahoo M. Standardization of quercetin in 2018;2018:575–8.
Pavun L. Spectrofluorimetric Determination Of Quercetin 2014;33:209–15.
Rahman MS, Ali MY, Ali MU. In Vitro Screening Of Two Flavonoid Compounds Isolated From Cassia Alata L . Leaves For Fungicidal Activities 2008:139–42.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 A. Mu'thi Andy Suryadi, Wicawati Van Solang, Mohamad Aprianto Paneo
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
